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Creators/Authors contains: "Chanderbali, Andre S."

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  1. Free, publicly-accessible full text available December 1, 2025
  2. Abstract Ancient whole-genome duplications (WGDs) characterize many large angiosperm lineages, including angiosperms themselves. Prominently, the core eudicot lineage accommodates 70% of all angiosperms and shares ancestral hexaploidy, termedgamma.Gammaarose via two WGDs that occurred early in eudicot history; however, the relative timing of these is unclear, largely due to the lack of high-quality genomes among early-diverging eudicots. Here, we provide complete genomes forBuxus sinica(Buxales) andTetracentron sinense(Trochodendrales), representing the lineages most closely related to core eudicots. We show thatBuxusandTetracentronare both characterized by independent WGDs, resolve relationships among early-diverging eudicots and their respective genomes, and use the RACCROCHE pipeline to reconstruct ancestral genome structure at three key phylogenetic nodes of eudicot diversification. Our reconstructions indicate genome structure remained relatively stable during early eudicot diversification, and reject hypotheses ofgammaarising via inter-lineage hybridization between ancestral eudicot lineages, involving, instead, only stem lineage core eudicot ancestors. 
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